Atividade antimicrobiana in vitro dos metabólitos produzidos pelo heliotropium indicum L. frente aos microrganismos escherichia colli,, staphylococcus aureus e proteus vulgaris

Abstract

Plants are used for various purposes, and is among the top its use as a medicine, due to this property the plants have become the subject of study from the laboratories of pharmaceutical industries and universities for the isolation of new active principles, which can be used in the production of new pharmaceuticals. Moreover, a large number of people use medicinal plants as a therapeutic resource, either by choice or because this is your only recourse. Therefore the present study was to evaluate the sensitivity of micro-organisms Escherichia coli, Staphylococcus aureus and Proteus vulgaris, compared to metabolites produced by species Heliotropium indicum L, obtained from these different techniques for different extraction technique metabolites. The Gathering was held at the abial neighborhood in the city of Tefé-AM, they were dehydrated will 350C for 24 hours, being crushed in industrial blender, For every 30 grams of crushed material was added 250 ml of 70% ethanol solvent, using as Soxhlet extractor. To the cold extract was used the same proportions of biological material and solvent allowed to stand for a period of 48 hours in a container âmbar.As dilutions of the extracts were used for both 0.2 μg and diluted in 20μl of DMSO and subsequently plus 1.96 ml distilled water, and were evaluated using the disc diffusion technique. The strains of E. coli and S. aureus and P.vulgaris were sown in Petri dishes containing nutrient medium 90x15mm Mueller Hinton.O experiment was performed in triplicate, was added at 80 μ l of microorganisms to be evaluated in Petri dishes and seeded with a 90x15mm handle Drigalsk. It was used the technique of diffusion disks ± 0.5 cm were used four disks per plate, were added in three 60 μ l of extract to be evaluated and fourth control group was added 60 μ l of DMSO as negative control later the plates were Boda temperature of 37 º C for 24 hours for observation of activity of the extract. Generally the extracts evaluated were effective against microorganisms Escherichia coli, and Staphylococcus aureus and Proteus vulgaris, should be noted however that the best results were against microorganisms Gram-negative Escherichia coli, that for both extraction techniques.