Produção de lacase por aspergillus brasiliensis a partir do resíduo da hidrodestilação de aniba canelilla e uso do extrato enzimático na descoloração de corantes

Abstract

The forest residues that are frequently dumped into the environment can be reused to obtain oxidative enzymes, reducing their environmental impact. These biocatalysts can be used in the industry to obtain various results such as the degradation of effluents dumped by the textile industry, which, without adequate care, have damaged animal and plant life. In this sense, the aim of this study was to produce laccase from the fungus Aspergillus brasiliensis using the hydrodistillation residue of leaves and branches of the species Aniba canilella (Lauraceae), for the use in the discoloration of dyes used in the textile industry. To obtain the residue, the hydrodistillation of leaves and branches of A. canelilla was carried out using a Clevenger apparatus. Subsequently, the residue was used as substrate for the cultivation of the fungus A. brasiliensis to obtain the laccase. By means of the enzymatic extract obtained, the discoloration of two synthetic dyes was carried out, the Brilliant Blue and Indigo Carmine dye. The variables analyzed for the determination of the best discoloration conditions were the pH, ranging from 4,0 and 6,0; the temperature, evaluated between 30 and 40ºC; and the variation of dye concentration from 0,5 to 1,0%. The highest percentage of discoloration were 7,0% for Brilliant Blue and 17.2%, for Indigo Carmine, obtained at pH 4.0, 40°C and 0.5% of dye concentration. With this study it was possible to use a lignocellulosic residue to obtain fungal laccase, and the fungal extract showed promise in discoloration of dyes used in the textile industry, which is responsible for high rates of environmental pollution.