Bioprospecção de fungos endofíticos de Minquartia guianensis Aubl. (OLACACEAE)

Abstract

This paper describes the search for bioactive substances from the study of chemical and biological crude extracts of Neosartorya hiratsukae endophytic fungi (Aspergillus anamorph) and Diaporthe hongkongensis (anamorph Phomopsis), which were isolated from the leaves of Minquartia guianensis. N. hiratsukae was grown on Sabouraud broth under the temperature of 25 ° C at 120 rpm in an incubator shaker for 25 days. D. hongkongensis was grown in Potato dextrose broth and Sabouraud under the temperature of 25 °C at 120 rpm in an incubator shaker at 20 and 25 days, respectively. The mycelium and extraction of the metabolic environment was taken with the organic solvent DCM, EtOAc and MeOH, resulting in crude extracts after evaporation of the solvent. After analysis by plaque ¹H NMR CCDC and crude extracts were selected as the chemical profile and fractionated on a column of silica opened. The extracts of N. hiratsukae and D. hongkongensis showed characteristic signs for the presence of terpenes, aromatics and triglycerides. Despite the wealth of interesting substances it has not been possible due to low isolation of bulk. The crude extracts of both fungi were tested for evaluation of antibacterial, antifungal and toxic opposite A. salina. N. hiratsukae extracts were not active in any of the tests. The DCM extract from mycelium D. hongkongensis showed activity at concentrations of 0.8 and 0.4 mg / ml against Cryptococcus gattii. The acetate extract at the highest concentration (1000 mg / mL) inhibited 100% of Pseudomonas aeruginosa growth while the DCM phase inhibited 87.3%. Already DCM extract had a percentage inhibition of 69% compared to Escherichia coli. These same samples when tested in a lower dose (500 ug / ml) had a significant reduction for inhibition. In general, the isolated endophytes of M. guianensis leaves showed interesting biotechnological potential due to production of secondary metabolites with antifungal activity.