Secreção de lacase utilizando a cepa amazônica trametes lactinea , suplementado com o resíduo da casca do astrocaryum aculeatum meyer, coletados no município de Alvarães /AM

Abstract

The group enzymes oxiredutases have potential applicability especially in industrial areas such as bioremediation, food industry and in the pulp and paper industry. In this context, and considering the fungal biodiversity of the Middle Solimões is essential to evaluate the enzymatic potential of these microorganisms. Few or almost no study has been done in this connection with fungi that region, hence the interest of evaluating the enzymatic potential of this diversity. Among the group of enzymes oxiredutases (ligninolytic) are mainly laccases, ligninases and peroxidases which have great potential for industrial use. These enzymes are produced by fungi belonging to the Basidiomycetes classes, a group that exhibits good representativity in the region. For the realization of this research, the collection of basidiocarps was held in March 2011, during the summer in a rural area of the municipality of Alvarães-AM. For isolation fungal used the synthetic medium BDA being removed fungal three fragments of approximately 5mm diameter edges of the plates and transferred to culture medium composed of 1000 ml of sterile distilled water, 1 g of glucose and peel flour Astrocaryum of aculeatum meyer concentration 10g / L erlenmeyer with a capacity of 150 ml, which were subsequently incubated in an environmental chamber in the stationary condition at a temperature of 30 ° C. The laccase of Trametes lactinea strain was at a concentration of 2% flour bark Astrocaryum aculeatum meyer and quantified in periods of 03, 06 and 09 days. To determine the activity of the enzyme was used device spectrophotometer absorbance reading of samples to calculate the laccase, we used the methodology proposed by Ander and Eriksson (1976). The method consists in the oxidation of syringaldazin enzyme substrate to form quinone which has absorption at 525 nm (ɛΣ = 65,000 M-1 cm -1). The strain Amazon Tramets lactinea expressed an excellent enzyme kinetics on the third day, thus underlining the interaction carbon source and assessed strain, it is noteworthy however that his greatest activity was the ninth day getting an enzymatic peak of 33.7 U / L.