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dc.contributor.authorRoessing, Daniel Saraiva-
dc.date.available2024-02-22-
dc.date.available2024-02-22T20:01:49Z-
dc.date.issued2023-12-15-
dc.identifier.urihttp://repositorioinstitucional.uea.edu.br//handle/riuea/5537-
dc.description.abstractThis dissertation describes a study that aimed to isolate and identify ligninolytic fungi from the Bosque da Ciência of the National Institute of Amazonian Research (INPA), located in Manaus, Amazonas, Brazil, and to evaluate their capacity for degrading low-density polyethylene (LDPE) films in agar medium and liquid medium. Thirty-eight samples of sporomes of ligninolytic macrofungi associated with decomposing lignocellulosic samples were collected. From these samples, eight fungi were isolated, four belonging to the phylum Basidiomycota and four to the phylum Ascomycota, with the latter four being contaminants of Basidiomycota. Phylogenetic analysis based on the ITS region sequence revealed precise identification of the isolated species, which were identified as Schizophyllum commune, Irpex laceratus, Phlebiopsis flavidoalba, Peniophora crassitunicata, Endomelanconiopsis endophytica, Annulohypoxylon stygium, Xylaria heliscus, and Xylaria plebeja. Regarding the results of LDPE film degradation assessment, all isolates showed potential in plastic degradation, depending on the culture conditions. In mineral agar medium, after 21 days of stationary culture at room temperature, all isolates except Xylaria plebeja XPM090323 promoted plastic mass reduction, with the most effective being Pleurotus ostreatus CPOINPA1, used as positive control (3.7%), Irpex laceratus ILM050722 (3%), Endomelanconiopsis endophytica EEM140223 (3.2%), and Annulohypoxylon stygium ASS170223 (3.8%). E. endophytica EEM140223 exhibited the highest laccase activity (18.1 U/Kg), while Schizophyllum commune SCM050722 excelled in peroxidase activity (5.9 U/Kg). In contrast, in liquid mineral broth, after 21 days of stationary culture at room temperature, X. plebeja XPM090323 stood out as the most efficient in reducing LDPE mass (1.5%). Finally, the isolate Xylaria heliscus XHS310123 presented the highest biomass production (4.1 g/L) and peroxidase activity (13.3 U/L), while S. commune SCM050722 showed the highest laccase activity (25.2 U/L). This study contributes to the understanding of the diversity of ligninolytic fungi in the Amazon region and their capacity for polyethylene degradation, presenting potential relevance in environmental applications and plastic waste remediationpt_BR
dc.languageporpt_BR
dc.publisherUniversidade do Estado do Amazonaspt_BR
dc.rightsAcesso Abertopt_BR
dc.subjectBasidiomycotapt_BR
dc.subjectAscomycotapt_BR
dc.subjectBiodegradaçãopt_BR
dc.subjectPlástico LDPEpt_BR
dc.subjectFeneloxidasespt_BR
dc.titleIdentificação, filogenia e biodegradação de plástico LDPE por fungos lignícolas isolados do Bosque do INPA (Basidiomycota Ascomycotapt_BR
dc.title.alternativeIdentification, phylogeny and biodegradation of LDPE plastic by lignicultural fungi isolated from the INPA Forest (Basidiomycota Ascomycotapt_BR
dc.typeDissertaçãopt_BR
dc.date.accessioned2024-02-22T20:01:49Z-
dc.contributor.advisor-co1Souza, João Vicente Braga de-
dc.contributor.advisor-co1Latteshttp://lattes.cnpq.br/7804981785557071pt_BR
dc.contributor.advisor1Souza, Érica Simplício de-
dc.contributor.advisor1Latteshttp://lattes.cnpq.br/4333531513081697pt_BR
dc.contributor.referee1Souza, Érica Simplício de-
dc.contributor.referee1Latteshttp://lattes.cnpq.br/4333531513081697pt_BR
dc.contributor.referee2Rocha, Waldireny Caldas-
dc.contributor.referee3Silva, João Paulo Alves-
dc.creator.Latteshttp://lattes.cnpq.br/0003379364095258pt_BR
dc.description.resumoEsta dissertação descreve um estudo que teve como objetivo isolar e identificar fungos lignícolas do Bosque da Ciência do Instituto Nacional de Pesquisas da Amazônia (INPA), localizado em Manaus, Amazonas, Brasil, e avaliar sua capacidade de degradação de filmes de polietileno de baixa densidade (LDPE) em meio com ágar e em meio líquido. Foram coletadas 38 amostras de esporomas de macrofungos ligninolíticos associados a amostras lignocelulósicas em decomposição. A partir dessas amostras, foram isolados oito fungos, sendo quatro pertencentes ao filo Basidiomycota e quatro ao filo Ascomycota, sendo estes quatro últimos contaminantes dos Basidiomycota. A análise filogenética baseada na sequência da região ITS revelou a identificação precisa das espécies dos isolados, que foram identificadas como Schizophyllum commune, Irpex laceratus, Phlebiopsis flavidoalba, Peniophora crassitunicata, Endomelanconiopsis endophytica, Annulohypoxylon stygium, Xylaria heliscus e Xylaria plebeja. Quanto aos resultados da avaliação da degradação de filmes de LDPE, todos os isolados demonstraram potencial na degradação do plástico, dependendo das condições de cultivo. Em meio com ágar mineral, após 21 dias de cultivo estacionário em temperatura ambiente, todos os isolados salvo Xylaria plebeja XPM090323 promoveram a redução da massa do plástico, destacando-se Pleurotus ostreatus CPOINPA1, usado como controle positivo (3,7%), Irpex laceratus ILM050722 (3%), Endomelanconiopsis endophytica EEM140223 (3,2%) e Annulohypoxylon stygium ASS170223 (3,8%). E. endophytica EEM140223 exibiu a atividade de lacase mais elevada (18,1 U/Kg), enquanto Schizophyllum commune SCM050722 se destacou na atividade de peroxidases (5,9 U/Kg). Em contraste, em meio líquido caldo mineral, após 21 dias de cultivo estacionário em temperatura ambiente, X. plebeja XPM090323 destacou-se como o mais eficiente na redução da massa do LDPE (1,5%). Por fim, o isolado Xylaria heliscus XHS310123 apresentou a maior produção de biomassa (4,1 g/L) e de peroxidases (13,3 U/L), enquanto S. commune SCM050722 apresentou a maior atividade de lacase (25,2 U/L). Este estudo contribui para a compreensão da diversidade de fungos lignícolas na região amazônica e sua capacidade de degradação de polietileno, apresentando potencial relevância em aplicações ambientais e de remediação de resíduos plásticospt_BR
dc.publisher.countryBrasilpt_BR
dc.publisher.programPPGMBT - PROGRAMA DE PÓS-GRADUAÇÃO EM BIOTECNOLOGIA E RECURSOS NATURAIS DA AMAZÔNIApt_BR
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