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dc.contributor.authorSousa, Milane Araújo de-
dc.date.available2023-11-08-
dc.date.available2023-11-21T13:00:55Z-
dc.date.issued2023-07-28-
dc.identifier.urihttp://repositorioinstitucional.uea.edu.br//handle/riuea/5338-
dc.description.abstractPolycythemia Vera (PV) is part of the group of myeloproliferative neoplasms (PMNs), in which it is characterized by an increase in erythrocyte mass. It is believed that VMs play an important role in the development of neoplasms and may be essential for understanding these pathologies. It has already been described that microvesicles (MVs) contribute to the development of PV with their greater procoagulant activity, increasing the risk of thrombotic complications. Objectives: To characterize the profile of circulating microvesicles in patients diagnosed with Polycythemia Vera treated at the Hospital Foundation for Hematology and Hemotherapy of Amazonas. Material and methods: Sixty-six individuals of both sexes were included and categorized into two groups: 33 patients with a conclusive diagnosis of Polycythemia Vera undergoing treatment and 33 healthy individuals for the control group. Clinical data were obtained from the patients' charts. Laboratory data were obtained from blood samples. Immunophenotypic characterization was performed on a CytoFlex S flow cytometer (Beckman Coulter) using the VM immunophenotyping protocol of the Integrated Group for Biomarker Research (GIPB) of the René Rachou Institute (IRR)-Fiocruz Minas. Results: There was a higher level of MVs CD34, CD13, CD33 (progenitor/myeloid), CD11c (dendritic cells), CD16, CD66b (neutrophils), CD3 (T Lymphocytes), CD19 (B Lymphocytes), CD56 (NK cells) , CD235a (erythrocytes) and CD51/61 (endothelial cells) in patients with PV. There were no significant levels of MVs in the groups of patients with and without thrombotic events. Higher levels of CD3 (lymphocytes) and CD51/61 (endothelial cells) MVs were identified in JAK2V617F+ patients with thrombotic events. Conclusion: In the analysis of the profile of MVs, higher levels of MVs were observed in patients with PV than in healthy individuals. It was also observed that JAK2V617F+ patients who have a history of thrombosis had higher levels of CD3 MVs (T lymphocytes) and CD51/61 (endothelial cells). in the pathogenesis of thrombosis. Future studies are essential to analyze VMs in the context of treatment, in addition to seeking to understand their role in the prognosis, as well as in the prothrombotic state of patients with PV.pt_BR
dc.languageporpt_BR
dc.publisherUniversidade do Estado do Amazonaspt_BR
dc.rightsAcesso Abertopt_BR
dc.subjectNeoplasia mieloproliferativapt_BR
dc.subjectMicrovesículapt_BR
dc.subjectPolicitemia verapt_BR
dc.subjectMyeloproliferative neoplasmpt_BR
dc.titleCaracterização do perfil de microvesículas cirdulantes em pacientes com policitemia verapt_BR
dc.title.alternativeCharacterization of the profile of circulating microvesicles in patients with polycythemia verapt_BR
dc.typeDissertaçãopt_BR
dc.date.accessioned2023-11-21T13:00:55Z-
dc.contributor.advisor-co1Mourão, Lucivana Prata de Souza-
dc.contributor.advisor-co1Latteshttp://lattes.cnpq.br/1135734404648095pt_BR
dc.contributor.advisor1Tarragô, Andréa Monteiro-
dc.contributor.advisor1Latteshttp://lattes.cnpq.br/4644326589690231pt_BR
dc.contributor.referee1Tarragô, Andréa Monteiro-
dc.contributor.referee1Latteshttp://lattes.cnpq.br/4644326589690231pt_BR
dc.contributor.referee2Silva Neto, Pedro Vieira da-
dc.contributor.referee2Latteshttp://lattes.cnpq.br/8932805410196413pt_BR
dc.contributor.referee3Oliveira, Sâmela Silva de-
dc.description.resumoA Policitemia Vera (PV) faz parte do grupo de neoplasias mieloproliferativas (NMPs), na qual é caracterizada pelo aumento da massa eritrócitária. Acredita-se que as MVs possuem um importante papel no desenvolvimento das neoplasias e podem ser essenciais para entendimento dessas patologias. Já foi descrito que as microvesículas (MVs) contribuem no desenvolvimento da PV com sua maior atividade pró-coagulante, aumentando o risco das complicações trombóticas. Objetivos: Caracterizar o perfil das Microvesículas circulantes em pacientes com diagnóstico de Policitemia Vera atendidos na Fundação Hospitalar de Hematologia e Hemoterapia do Amazonas. Material e métodos: Sessenta e seis indivíduos, de ambos os sexos, foram incluídos e categorizados em dois grupos: 33 pacientes com diagnóstico conclusivo para Policitemia Vera em tratamento e 33 indivíduos saudáveis para o grupo controle. Dados clínicos foram obtidos dos prontuários dos pacientes. Dados laboratoriais foram obtidos de amostras de sangue. A caracterização imunofenotípica foi realizada no citômetro de fluxo CytoFlex S (Beckman Coulter) através do protocolo de imunofenotipagem de MVs do Grupo Integrado em Pesquisas de Biomarcadores (GIPB) do Instituto René Rachou (IRR)-Fiocruz Minas. Resultados: Observou-se maior nível de MVs CD34, CD13, CD33 (progenitoras/mieloides), CD11c (células dendríticas), CD16, CD66b (neutrófilos), CD3 (Linfócitos T), CD19 (Linfócitos B), CD56 (células NK), CD235a (eritrócitos) e CD51/61 (células endoteliais)em pacientes com PV. Não foi apresentando níves significativos de MVs nos grupos de pacientes com e sem eventos trombóticos. Foi identificado níves maiores de MVs CD3 (linfócitos) e CD51/61 (células endoteliais) em pacientes JAK2V617F+ com eventos trombóticos. Conclusão: Na análise do perfil de MVs foi observado níveis maiores de MVs em pacientes com PV do que os indíviduos saudáveis. Foi observado também que os pacientes JAK2V617F+ que apresentam histórico de trombose apresentaram níves maiores de MVs CD3 (linfócitos T) e CD51/61 (células endoteliais), esses resultados mostram que a variante genética JAK2V617F possui um papel importante no desencadeamento das MVs e que favoreçem na patogênese da trombose. Estudos futuros são essenciais para analisar as MVs no contexto do tratamento, além de buscar entender o seu papel no prognóstico, assim como, no estado pró-trombótico de pacientes com PVpt_BR
dc.publisher.countryBrasilpt_BR
dc.publisher.programPPGH -PROGRAMA DE PÓS-GRADUAÇÃO EM CIÊNCIAS APLICADAS À HEMATOLOGIApt_BR
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dc.publisher.initialsUEApt_BR
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