Estudo químico dos extratos de Kalanchoe pinnata (lam.) pers. nativa do município de Tabatinga AM

Abstract

Kalanchoe pinnata formerly known as Bryophyllum pinnatum, is a herbaceous species native to tropical regions such as Africa. In Brazil, it was successfully introduced and propagated, being commonly used in communities far from large capitals. The juice of the leaves is often used to treat diseases inflammatory conditions, gastric ulcers, burns, diarrhea, vomiting, insect bites and body pain. In the literature, compounds such as anthraquinones and flavonoids are reported to have different biological activities such as antimicrobial, antioxidant, cytotoxic, antitumor, antiparasitic, anti-allergic and hepatoprotective. Based on this information and considering that there are few studies on the chemical compounds of K. pinnata in Amazonas, mainly in regions further away from the capital, such as in the municipality of Tabatinga. The present work aimed to determine the chemical profile of the fractions of the crude extract of K. pinnata native to the municipality of Tabatinga - AM. For this, plant material was collected from a local producer in the municipality of Tabatinga. Then, the leaves were subjected to infusion extraction. Soon after, the crude extract obtained was fractionated with hexane, chloroform and ethyl acetate. The resulting fractions were analyzed by High Performance Liquid Chromatography (HPLC) at wavelength λ=254 nm and, using the information available in the literature, it was possible to establish a relationship between the quercetin compound 3-O-α-L-arabinopyranosyl- (1→2)-α-L-rhamnopyranoside, as responsible for the chromatographic peaks observed in the hexane, chloroform and ethyl acetate fractions. After Mass Spectrometry (MS) analysis, it was possible to propose four compounds: isorhamnetin-3-rutinoside, rhamnosyl hexosyl methyl quercetin, quercitrin and physcion. All with antioxidant properties, as indicated in the literature. In a biological test for antioxidant potential, the chloroform fraction stood out. Thus, the present study will serve as a basis for future work to characterize chemical compounds present in K. pinnata from the municipality of Tabatinga.