Prospecção de naftoquinonas em culturas de Quambalaria sp. e sua avaliação como agente citotóxico

Abstract

The manipulation and keeping of endophytic fungus in laboratory propitiates the development of new drugs in viable way, as aspects economics than ecological, once there is not necessity of seedlings use. The endophytic fungi isolated by palm tree fruit, popularly known as açaí-da amazônia (Euterpe precatoria), denominated Quambalaria sp., is naphthoquinone producer with biologics activities, being them quambalarine A, mompain and quambalarine B, them demonstrated antimicrobial activity along with other anticancer activity. Seeing that biotechnology activity, this study aims to prospect and characterize naphthoquinones, further evaluate the cytotoxic activity. Therefore, the Quambalaria sp. submitted by OSMAC technique, wich consisted in the use of different mediums culture. The choose mediums culture were extracted with organic solvents and this analyzed by the HPLC/MS method. After analysis, naphthoquinones were characterize and tested extract in cells hepatics (Huh-7) cytotoxicity activity. The fungi had better growth and naphthoquinones production in culture medium control PD (Potato Dextrose), exhibited in extract non-cytotoxic for hepatics cells in the concentrations 50 μg/ml, 10 μg/ml and 1 μg/ml. Keywords: Quambalaria sp., naphthoquinones; quambalarine B; cytotoxicity.