DSpace logo

Use este identificador para citar ou linkar para este item: http://repositorioinstitucional.uea.edu.br//handle/riuea/2376
Registro completo de metadados
Campo DCValorIdioma
dc.contributor.authorOjeda, Cinthya Paola Ortiz-
dc.date.available2020-03-18-
dc.date.available2020-03-19T00:26:08Z-
dc.date.issued2017-03-18-
dc.identifier.urihttp://repositorioinstitucional.uea.edu.br//handle/riuea/2376-
dc.description.abstractThe Amazon rainforest has great biodiversity of species, including animals, plants and microorganisms. Many of these microorganisms are not yet known, as well as some of the potential uses and properties of these. Among these are the endophytic microorganisms who live inside the plants, many of them showing the same properties of the host. The plant “jambu” Acmella ciliata is known for its antimicrobial, antifungal properties among others, and is used in food and natural medicine in the Amazon region. For the isolation of endophytes, first held low-level disinfection of the twigs and leaves of the plant Acmella ciliata, sow and incubate the pieces of this plant material during 15 days at 28ºc. The isolated microorganisms were evaluated in trials of antagonism (Fusarium decemcellulare and Colletotrichum gloeosporioides) and antimicrobial activity (Streptococcus pyogenes and Candida parapsilosis). A total of 104 were isolated endophytic microorganisms (bacteria, filamentous fungi and yeasts), of this universe, 22 filamentous fungi and 16 bacteria showed inhibition of growth of plant pathogens, and six bacteria showed antimicrobial activity for Streptococcus pyogenes and Candida parapsilosis. Was shown that the endophytic microorganisms isolated from plant Acmella ciliata produce metabolites with antimicrobial and antifungal activity.pt_BR
dc.languageporpt_BR
dc.publisherUniversidade do Estado do Amazonaspt_BR
dc.rightsAcesso Abertopt_BR
dc.rightsAtribuição-NãoComercial-SemDerivados 3.0 Brasil*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/br/*
dc.subjectendofíticopt_BR
dc.subjectjambupt_BR
dc.titlePotencial Biotecnológico dos microrganismos endofíticos isolados de plantas de jambu (Acmella ciliata (Kunth) Cass.) para controle de microrganismos patógenospt_BR
dc.typeDissertaçãopt_BR
dc.date.accessioned2020-03-19T00:26:08Z-
dc.contributor.advisor-co1Andrade, Suanni Lemos de-
dc.contributor.advisor-co1Latteshttp://lattes.cnpq.br/7870482573275809pt_BR
dc.contributor.advisor1Procópio, Rudi Emerson de Lima-
dc.contributor.advisor1Latteshttp://lattes.cnpq.br/2478199435796976pt_BR
dc.contributor.referee1Procópio, Rudi Emerson de Lima-
dc.contributor.referee1Latteshttp://lattes.cnpq.br/7870482573275809pt_BR
dc.creator.Latteshttp://lattes.cnpq.br/0881701657428137pt_BR
dc.description.resumoA floresta amazônica possui grande biodiversidade de espécies, entre animais, plantas e microrganismos. Muitos destes microrganismos ainda não são conhecidos assim como os potenciais usos e propriedades destes. Entre estes se encontram os microrganismos endofíticos que habitam no interior das plantas, muitos deles apresentando as mesmas propriedades dos hospedeiros. A planta “jambu” Acmella ciliata é conhecida por suas propriedades antimicrobianas, antifúngicas entre outras, e é usada na gastronomia e na medicinal natural na região Amazônica. Para o isolamento dos endófitos, primeiro foi realizada a desinfecção superficial dos galhos e folhas da planta Acmella ciliata, para semear e incubar posteriormente os pedaços deste material vegetal durante 15 dias a 28ºC. Os microrganismos isolados foram avaliados em ensaios de antagonismo (Fusarium decemcellulare e Colletotrichum gloeosporioides) e atividade antimicrobiana (Streptococcus pyogenes e Candida parapsilosis). Foram isolados um total de 104 microrganismos endofíticos (fungos filamentosos, bactérias e leveduras), deste universo, 22 fungos filamentosos e 16 bactérias apresentaram inibição de crescimento dos fitopatógenos, e seis bactérias apresentaram atividade antimicrobiana para Streptococcus pyogenes e Candida parapsilosis. Evidenciou-se que os microrganismos endofíticos isolados da planta Acmella ciliata produzem metabólitos com atividade antimicrobiana e antifúngica.pt_BR
dc.publisher.countryBrasilpt_BR
dc.publisher.programPrograma de Pós-Graduação em Biotecnologia e Recursos Naturaispt_BR
dc.relation.referencesAili T., Fahu P., Siliang H., Gongming Y., Bin L., Tan W. (2014) Characterisation of endophytic Bacillus thuringiensis strains isolated from wheat plants as biocontrol agents against wheat flag smut. Biocontrol Science and Technology, 24 (8) 901-924. Anisha C., Radhakrishnan E.K. (2015) Gliotoxin-producing endophytic Acremonium sp. from Zingiber officinale found antagonistic to soft rot pathogen Pythium myriotylum. Applied Biochemestry and Biotechnology, 175 (7) 3458-3467. Arora S., Vijay S., Kumar D. (2011) Phytochemical and antimicrobial studies on the leaves of Spilanthes acmella. Journal of Chemical and Pharmaceutical Research 3 (5) 145-150. Azevedo J. L. (1998) Microrganismos endofíticos. Em: Melo I. S., Azevedo J. L. (Ed.) Ecologia microbiana. Jaguariúna: EMBRAPA, pp. 117-137. Azevedo J.L., Maccheroni W.J., Pereira J.O., Araújo W.L. (2000) Endophytic microrganisms: a review on insect control and recent advances on tropical plants. Electronic Journal of Biotechnology, 3 (1) 15-16. Badalyan S.M., Innocenti G., Garibyan N.G. (2002) Antagonistic activity of xylotrophic mushrooms against pathogenic fungi of cereals in dual culture. Phytopathologia Mediterranea, Bologna, 41 (3) 200-225. Banhos E.F. dos, Souza A.Q.L. de, Andrade J.C. de, Souza A.D. de, Koolen H.H.F., Albuquerque P.M. (2014) Endophytic fungi from Myrcia guianensis at the Brazilian Amazon: Distribution and bioactivity. Brazilian Journal of Microbiology, 45 (1) 153-161. 59 Bezerra J.D.P., Nascimento C.C.F., Barbosa R.do N., Silva D.C.V. da, Svedese V.M., Silva-Nogueira E., Gomes B.S., Paiva L.M., Souza-Motta C.M. (2015) Endophytic fungi from medicinal plant Bauhinia forficata: Diversity and biotechnological potential. Brazilian Journal of Microbiology, 46 (1) 49-57. Bogas A.C., Ferreira A.J., Araújo W.L., Astolfi-Filho S., Kitajima E.W., Lacava P.T., Azevedo J.L. (2015) Endophytic bacterial diversity in the phyllosphere of Amazon Paullinia cupana associated with asymptomatic and symptomatic anthracnose. Springerplus, 4: 258. Borate P.P., Disale S.D. (2013) Studies on antibacterial activity of Acmella oleracea (L.) Murr. International Journal of Pharmaceutical Science and Health Care, 3 (5) 36-42. Campanile G., Ruscelli A., Luisi N. (2007) Antagonistic activity of endophytic fungi towards Diplodia corticola assessed by in vitro and in plant test. European Journal of Plant Pathology, 117 (3) 237-246. Canuto K., Rodrigues T., Oliveira de F., Gonçálves F. (2012) Embrapa. Fungos endofíticos: Perspectiva de descoberta e aplicação de compostos bioativos na agricultura. Documentos 154. Brasilia, DF. Castillo U. F., Strobel G. A., Ford E. J., Hess W. M., Porter H., Jensen J. B., Albert H., Robison R., Condron M. A. M., Teplow D. B., Stevens D., Yaver D. (2002) Munumbicins, wide-spectrum antibiotics produced by Streptomyces NRRL 30562, endophytic on Kennedia nigriscans. Microbiology, 148 (9) 2675-2685. Chan Y-K., McCormik W.A., Seifer K.A. (2003) Characterization of an antifungal soil bacterium and its antagonistic activities against Fusarium species. Canadian Journal of Microbiology, 49 (4) 253-262. 60 Chithra S., Jasmin B., Sachidanandan P., Jyothis M., Radhakrishnan E.K. (2014) Piperine production by endophytic fungus Colletotrichum gloeosporioides isolated from Piper nigrum. Phytomedicine, 21 (4) 534-540. Christina A., Christapher V., Bhore S.J. (2013) Endophytic bacteria as a source of novel antibiotics: An overview. Pharmacognosy Reviews, 7 (13) 11-16. Chung K.F., Kono Y., Wang C.-M., Peng, C.-I. (2008) Notes on Acmella (Asteraceae): Helianthaceae in Taiwan. Botanical Studies, 49 (1) 73-82. Daisy M.J., Raju A.R., Subin M.P. (2013) Qualitative phytochemical analysis and in vitro antibacterial activity of Acmella ciliata (H.B.K.) Cassini and Ichnocarpus frutescens (Linn.) R.Br. against two pathogenic bacteria. Nature Environment and Pollution Technology, 12 (1) 167-170. Das K.K. (2013) A new distributional record of Acmella ciliata (Kunth) Cassini (Asteraceae) from Assam, India. Pleione, 7 (1) 258-261. Dhanya N.N., Padmavathy S. (2014) Impact of endophytic microorganisms on plants, environment and humans. Review article. The Scientific World Journal, vol. 2014, Article ID 250693, 11 pages. Ding L., Maier A., Fiebig H.H., Lin W.H., Hertweck C. (2011) A family of multicyclic indolosesquiterpenes from a bacterial endophyte. Organic and Biomolecular Chemistry, 9 (11) 4029-4031. Duarte M.C.T., Figueira G.M., Sartoratto A., Rehder V.L.G., Delarmelina C. (2005) Anti-Candida activity of Brazilian medicinal plants. Journal of ethnopharmacology, 97 (2) 305-311. Fuga C.A.G., Gonçalves D.C., Cunha W.V. (2011) Inibição do crescimento micelial de Colletotrichum gloeosporioides por Bacillus spp. ”in vitro”. Revista do Núcleo Interdisciplinar de Pesquisa e Extensão, 1 (8) 188-194. 61 Hanada R.E., Pomella A.W.V., Costa H.S., Bezerra J.L., Loguercio L.L., Pereira J.O. (2010). Endophytic fungal diversity in Theobroma cacao (cacao) and T. grandiflorum (cupuaçu) trees and their potential for growth promotion and biocontrol of black-pod disease. Fungal Biology, 114 (11-12) 901-910. Harrison L., Teplow D. B., Rinaldi M., Strobel G. (1991) Pseudomycins, a family of novel peptides from Pseudomonas syringae possessing broad-spectrum antifungal activity. Journal of General Microbiology, 137 (12) 2857-2865. Jalgaonwala R.E., Mohite B.V., Mahajan R.T. (2010) Evaluation of endophytes for their antimicrobial activity from indigenous medicinal plants belonging to North Maharashtra region India. International Journal on Pharmaceutical and Biomedical Research, 1 (5) 136-141. Jansen R.K. (1985). The systematics of Acmella (Asteraceae-Heliantheae). Systematic Botany Monographs, 1-115. Jasim B., Mathew J., Radhakrishnan E. K. (2016) Identification of a novel endophytic Bacillus sp. from Capsicum annuum with highly efficient and broad spectrum plant probiotic effect. Journal of Applied Microbiology, 121 (4) 1079-1094. Kawamoto S.O., Lorbeer J.W. (1976) Protection of onion seedling from Fusarium oxysporum f. sp. cepae by seed and soil infestation with Pseudomonas cepacia. Plant Dissease, 60: 189–191. Kern M.E., Blevins K.S. (1999) Micologia médica – Texto e Atlas. 2. ed, São Paulo: Editoral Premier. Lacava P., Li W., Araújo W., Azevedo J., Hartung J. (2006) Rapid, specific and quantitative assays for the detection of the endophytic bacterium 62 Methylobacterium mesophilicum in plants. Journal of Microbiological Methods, 65 (3) 535-541. Li X., Zhang Q., Zhang A., Gao J. (2012) Metabolites from Aspergillus fumigatus, an endophytic fungus associated with Melia azedarach, and their antifungal, antifeedant, and toxic activities. Journal of Agricultural and Food Chemistry, 60 (13) 3424-3431. Ma L., Cao Y.H., Cheng M.H., Huang Y., Mo M.H., Wang Y., Yang J.Z., Yang F.X. (2013) Phylogenetic diversity of bacterial endophytes of Panax notoginseng with antagonistic characteristics towards pathogens of root-rot disease complex. Antonie van Leeuwenhoek, 103 (2) 299-312. Maki C.S. (2006). Diversidade e potencial biotecnológico de fungos endofíticos de cacau (Theobroma cacao L.). Tese para obter o título de Doutor em Agronomia. Área de concentração: Genética e Melhoramento de Plantas. Universidade de São Paulo, Escola Superior de Agricultura “Luiz de Queiroz”. Piracicaba. pp. 127. Mapperson R.R., Kotiw M., Davis R.A., Dearnaley J.D.W. (2014) The diversity and antimicrobial activity of Preussia sp. endophytes isolated from australian dry rainforest. Current Microbiology, 68 (1) 30-37. Mello V.K. de, Medeiros G.L., Diniz J.M.P., Stringari D., Terasawa L.V.G, Jaccoud Filho D. de S., Matiello, R.R., Rudnik, S., Pileggi, M., Pileggi, S.A.V. (2010). Antagonismo in vitro e obtenção dos extratos brutos de microrganismos endofíticos isolados do milho crioulo frente ao fungo fitopatogênico Fusarium sp. I51-2. I Congresso de Iniciação Científica de Pós-Graduação – Florianópolis (SC). Monarco C., Sisterna M., Perelló A., Bello D.G. (2004) Preliminary studies on biological control of the Blackpoint Complex of wheat in Argentina. World Journal of Microbiology e Biotechnology, 20 (3) 285-290. 63 Moreno S.C., Carvalho G.A., Picanço M.C., Morais E.G.F., Pereira R.M. (2012) Bioactivity of compounds from Acmella oleraea against Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae) and selectivity to two non-target species. Pest Management Science, 68 (3) 386-393. Oliveira K.M. de, Boas E.V., Bonett L.P., Cardozo Júnior E.L., Bernardi-Wenzel J. (2015) Isolamento e atividade antibacteriana de fungos endofíticos de Piper glabratum Kunth. Arquivos de Ciência e Saúde UNIPAR, Umuarama, 19 (1) 3-9. Organización Mundial de la Salud (OMS) (2016) Nota descriptiva: Resistencia a los antibióticos. Octubre 2016 http://www.who.int/mediacentre/factsheets/antibiotic-resistance/es/ Peixoto-Neto P.A.D.S.P., Azevedo J.L., Araújo, W. L. (2002) Microrganismos endofíticos: interação com plantas e potencial biotecnológico. Biotecnologia: Ciência & Desenvolvimento, 29, 62-77. Petrini O. (1991). Fungal endophyte of tree leaves. En: Andrews J., Hirano S.S. (Eds). Microbial Ecology of Leaves. New York. Springer Verlag. pp.179-197. Phongpaichit S., Nikom J., Rungjindamai N., Sakayaroj J., Hutadilok-Towatana N., Rukachaisirikul V., Kirtikara K. (2007) Biological activities of extracts from endophytic fungi isolated from Garnicia plants. FEMS Immunol Med Microbiol, 51(3) 517- 525. Pimentel I., Glienke-Blanco C., Gabardo J., Makowiecky R., Azevedo J.L. (2006) Identification and Colonization of Endophytic Fungi from Soybean (Glycine max (L.) Merril) under Different Environmental Conditions. International Journal Brazilian Archives of Biology and Technology, 49 (5) 705-711. 64 Prachayasittikul S., Suphapong S., Worachartcheewan A., Lawung R., Ruchirawat S., Prachayasittikul V. (2009) Bioactive metabolites from Spilanthes acmella Murr. Molecules, 14 (2) 850-867. Rani S.A., Murty, S.U. (2006) Antifungal potential of flower head extract of Spilanthes acmella Linn. African Journal of Biomedical Research, 9 (1) 67-69. Rincón C.A.M., Castaño J.C.O., Ríos E.V. (2012) Actividad biológica de los aceites esenciales de Acmella ciliata (Kunth) Cass. Revista Cubana de Plantas Medicinales, 17 (2) 160-171. Rocha R., Eleutério D., Engels C., Veiga S.A., Jaccoud D., Rodrigues R., Pileggi M. (2009) Selection of endophytic fungi from comfrey (Symphytum officinale L.) for in vitro biological control of the phytopathogen Sclerotinia sclerotiorum (LIB.). Brazilian Journal of Microbiology, 40 (1) 73-78. Santi F.I.F., Machado Z.O., Soto H.H.G, Segal E.I.F., Ramos H.B. (2012) Endophytic and rhizospheric enterobacteria isolated from sugar cane have different potentials for producing plant growth-promoting substances. Plant Soil, 353 (1) 409-417. Santos T.T., Varavallo M.A. (2011) Aplicação de microrganismos endofíticos na agricultura e na produção de substâncias de interesse económico. Semina: Ciências Biológicas e da Saúde, Londrina, 32 (2) 199-212. Sbravatti J.A., Garcia C., Chapaval I., Figueredo A., Schultz B. (2013) Seleção in vitro de fungos enndofíticos para o controle biológicos de Botrytis cinerea em Eucalyptus benthamii. Floresta, Curitiba, PR, 43 (1) 145 – 152. 65 Selim K.A., El-Beih A.A., AbdEl-Rahman T.M., El-Diwany A.I. (2011) Biodiversity and antimicrobial activity of endophytes associated with Egyptian medicinal plants. Mycosphere 2 (6) 669-678. Silva M.P., Barbosa F.S.Q., Barros R. F. M. de (2014) Estudo taxonômico e etnobotânico sobre a família Asteraceae (Dumortier) em uma comunidade rural no Nordeste do Brasil. Gaia Scientia, 8 (2) 110-123. Silva M.C.S. (2015). Bioprospecção e caracterização de microrganismos endofíticos de isolados de sementes de guaranazeiro e o controle da antracnose (Colletotrichum spp.). Tese para obter o grau de Mestre em Ciências. Centro de Energia Nuclear na Agricultura da Universidade de São Paulo. Piracicaba. pp. 76. Sousa K.A. de, Orlanda J.F. de, Bezerra G., Sousa T. de (2013) Estudo do potencial de fungos endofíticos no controle do agente causal da fusariose em tomateiro. Agroecossistemas, 5 (1) 50-55. Souza A., Souza A., Astolfi Filho S., Belém Pinheiro M.L., Sarquis M.I., Pereira J. (2004) Atividade antimicrobiana de fungos endofíticos isolados de plantas tóxicas da amazônia: Palicourea longiflora (aubl.) rich e Strychnos cogens bentham. Acta Amazônica, 34 (2) 185-195. Souza R.D., Mendoça E.A.F., Soares M.A. (2015) Atividade antagônica a microrganismos patogênicos por bactérias endofíticas isoladas de Echinodorus scaber Rataj. Summa Phytopathologica, 41 (3) 229-232. Sun L., Lu Z., Bie X., Lu F., Yang S. (2006) Isolation and characterization of a co-producer of fengycins and surfactins, endophytic Bacillus amyloliquefaciens ES-2, from Scutellaria baicalensis Georgi. World Journal of Microbiology and Biotechnology, 22 (12) 1259-1266. 66 Thompson T., Sukesh K., Singh D. (2012) A study on the antimicrobial effect of Acmella oleraceae against dental caries bacteria. International Journal of Pharmaceutical Sciences and Research, 3 (4) 1194-1197. Trivedi P., Duan Y., Wang N. (2010) Huanglogbing, a systemic disease, restructures the bacterial community associated with citrus roots. Applied and Environmental Microbiology, 76 (11) 3427-3436. Vrije T., Antoine N., Buitelaar M.R., Bruckner S., Dissevelt M., Durand A., Gerlagh M., Jones E.E., Lüth P., Oostra J., Ravensberg W.J., Renaud R., Rinzema A., Weber F.J., Whipps J.M. (2001) The fungal biocontrol agent Coniothyrium minitans: Production by solid-state fermentation, application and marketing. Applied Microbiology e Biotechnology, 56 (1-2) 58-68. Wani M.A., Sanjana K., Kumar D.M., Lal D.K. (2010) Short Communication. GC-MS analysis reveals production of 2-phenylethanol from Aspergillus niger endophytic in rose. Journal of Basic Microbiology, 50 (1) 110-114. Wicklow D.T., Roth S., Deyrup S.T., Gloer J.B. (2005) A protective endophyte of maize: Acremonium zeae antibiotics inhibitory to Aspergillus flavus and Fusarium veticillioides. Mycological Research, 109 (5) 610-618. Zanardi L., Bolzani V., Cavalheiro A., Silva D.H., Trevisan H.C., Araujo A.R. (2012) Sesquiterpenos produzidos pelo fungo endofítico Phomopsis cassia com atividade antifúngica e inibidora de acetilcolinesterase. Quimica Nova, 35 (11) 2233-2236. Zhao J., Shan T., Mou Y., Zhou L. (2011) Plant-derived bioactive compounds produced by endophytic fungi. Mini-Reviews in Medicinal Chemistry, 11 (2) 159 - 168. Zhou J.Y., Zhao X.Y., Dai C.C. (2014) Antagonistic mechanisms of endophytic Pseudomonas fluorescens against Athelia rolfsii. Journal of Applied Microbiology, 117 (4) 1144-1158.pt_BR
dc.subject.cnpqbiotecnologiapt_BR
dc.publisher.initialsUEApt_BR
Aparece nas coleções:DISSERTAÇÃO - MBT Programa de Pós-Graduação em Biotecnologia e Recursos Naturais da Amazônia



Este item está licenciada sob uma Licença Creative Commons Creative Commons