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dc.contributor.authorAraújo, José Deney Alves de-
dc.date.available2020-03-16-
dc.date.available2020-03-17T18:05:02Z-
dc.date.issued2016-06-30-
dc.identifier.urihttp://repositorioinstitucional.uea.edu.br//handle/riuea/2322-
dc.description.abstractThousands of water bodies are found in the Amazon. They can be classified based on their water color in blackwater, clearwater and whitewater. The Amazon basin houses approximately 3,000 fish species, including the freshwater sardine, Triportheus albus, locally known as “sardinha”. Triportheus albus lives in all three types of water, despite their significant differences regarding physicochemical parameters. The ability of this species to survive in these different habitats is anticipated to be related to specific adaptations. The goal of the present study is to describe gene transcription differences of T. albus collected from the three types of water, and to describe the relevant mechanisms behind this ability. Gills of specimens of T. albus from the three types of water (black, clear, and white) were collected. Nine cDNA libraries, three biological replicates for each condition (type of water) were prepared and sequenced for RNA (RNA-Seq) using the MiSeq® (Illumina®) platform. A total of 51.6 million of reads paired-end, were assembled into 285,456 high quality contigs. Considering FDR ≤ 0.05 and the fold change ≥ 2, 13,754 differentially expressed genes were detected for all three conditions. Two mechanisms related to the homeostasis control were detected for T. albus living in blackwater. The acidic blackwater seems to be a challenging environment to many types of organisms. The first mechanism is related to a decrease of cell permeability and the second seem to be related to ion and acid-base regulation. We suggest that T. albus is an important fish species for future studies exploring ion and acid-base regulation in fish of the Amazon. Key words: Negro River, Tapajós River, Solimões River, differentially expressed genes, RNA-Seq, acid pH, ionic regulation.pt_BR
dc.languageporpt_BR
dc.publisherUniversidade do Estado do Amazonaspt_BR
dc.rightsAcesso Abertopt_BR
dc.rightsAtribuição-NãoComercial-SemDerivados 3.0 Brasil*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/br/*
dc.subjectRio Negropt_BR
dc.subjectRio Tapajóspt_BR
dc.subjectRio Solimõespt_BR
dc.subjectExpressão diferencialpt_BR
dc.subjectRNASeqpt_BR
dc.subjectpH ácidopt_BR
dc.subjectRegulação iônicapt_BR
dc.titleAnálise da expressão diferencial do transcriptoma da espécie Triportheus albus cope, 1872 nas águas preta, clara e branca da Amazôniapt_BR
dc.typeDissertaçãopt_BR
dc.date.accessioned2020-03-17T18:05:02Z-
dc.contributor.advisor-co1Ghelfi, Andrea-
dc.contributor.advisor-co1Latteshttp://lattes.cnpq.br/1250847135870845pt_BR
dc.contributor.advisor1Val, Adalberto Luis-
dc.contributor.advisor1Latteshttp://lattes.cnpq.br/2747150211073176pt_BR
dc.contributor.referee1Val, Adalberto Luis-
dc.contributor.referee1Latteshttp://lattes.cnpq.br/2747150211073176pt_BR
dc.contributor.referee2Alencar, Tainá Raiol-
dc.contributor.referee2Latteshttp://lattes.cnpq.br/9295686881191724pt_BR
dc.contributor.referee3Souza, Antonia Queiroz Lima de-
dc.contributor.referee3Latteshttp://lattes.cnpq.br/8499987875894209pt_BR
dc.creator.Latteshttp://lattes.cnpq.br/8028027637749067pt_BR
dc.description.resumoMilhares de corpos d’água são encontrados na Amazônia. Eles podem ser classificados conforme sua cor em três categorias, águas preta, clara e branca. Estima-se que 3.000 espécies de peixes vivem nos rios da Amazônia, dentre estas a sardinha, Triportheus albus. Esta espécie habita os três tipos de águas da Amazônia, apesar de suas diferenças significativas em relação aos parâmetros físico-químicos. A capacidade desta espécie para sobreviver nestes diferentes habitats está relacionada com suas adaptações específicas. O objetivo do presente estudo foi descrever a resposta gênica nos três tipos de águas, e descrever os mecanismos relevantes que podem originar essa capacidade. Brânquias de T. albus foram coletadas em locais bem caracterizados para cada tipo de água. Nove bibliotecas de cDNA foram construídas, três réplicas biológicas de cada condição e sequenciado o RNA (RNA-Seq) na Plataforma MiSeq® (Illumina®). Um total de 51,6 milhões de reads paired-end, e 285.456 transcritos foram montados. Considerando o FDR ≤ 0,05 e fold change ≥ 2, foram detectados 13.754 genes diferencialmente expressos nos três desafios ambientais. Dois mecanismos relacionados com a homeostase foram detectados em T. albus que vivem em águas pretas. As águas pretas e ácidas, parece ser um ambiente desafiador para muitos tipos de organismos aquáticos. O primeiro está relacionado com a diminuição da permeabilidade celular e o segundo com a regulação iônica e ácido-base. Sugerimos que a espécie T. albus é uma boa espécie de peixe para futuros estudos envolvendo a regulação iônica e ácido-base de espécies amazônicas. Palavras-chave: Rio Negro, Rio Tapajós, Rio Solimões, expressão diferencial, RNASeq, pH ácido, regulação iônica.pt_BR
dc.publisher.countryBrasilpt_BR
dc.publisher.programPrograma de Pós-Graduação em Biotecnologia e Recursos Naturaispt_BR
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dc.subject.cnpqBiotecnologiapt_BR
dc.publisher.initialsUEApt_BR
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