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dc.contributor.authorMuniz, Veranilce Alves-
dc.date.available2020-03-12-
dc.date.available2020-03-13T14:17:18Z-
dc.date.issued2019-04-22-
dc.identifier.urihttp://repositorioinstitucional.uea.edu.br//handle/riuea/2281-
dc.description.abstractThe Aedes aegypti mosquito is a vector of the arboviruses Dengue, Zika and Chikungunya, which cause public health problems in several regions of the world. Vector control is the best way to alleviate this problem, which may be socio-educational, physical, chemical and biological. However, chemicals cause negative impacts on the environment and humans. The use of entomopathogenic microorganisms, mainly bacteria of the genus Bacillus, becomes a promising alternative in the fight against this mosquito. The objective of this study was to select bacteria isolated from Amazonian environments, carrying the Cry and BS-glu genes, with larvicidal potential, to contribute to the control of Ae. aegypti. In this work 21 lines of collections of academic papers were obtained from soils, water, plant and insect from different Amazonian environments. The bacilli strains of this study were submitted to the PCR reaction using the nucleotide sequence encoding the rRNA16S gene. In total, 20 bacterial strains were identified, belonging to the following genera: Bacillus, Brevibacillus. Brevundimonas, Serratia and Achromobacter. PCR was then performed to characterize the Cry4Ba, Cry11 and BS-glu genes. The results showed that of the 21 lines analyzed, six lines - SPa09, SPa04, 15PHA, BtAM06, R22 and GD 02.13 - presented amplification for the Cry4Ba gene. Considering the BS-glu gene, two strains - SBC2 and standard strain Bti001- amplified for said gene. In the evaluation of the larvicidal activity the quantitative bioassays with 21 strains of bacilli against Ae. aegypti. The results of the quantitative bioassays showed that seven lines were promising in the bacterial biomass assays, where five - R22ISP2, GDO2.13NA, BtAM06, BtAM49LB and SPa09NA - presented 100% mortality in all concentrations - 133 mg / L, 66.6 mg / L, 33.3 mg / L, 16.6 mg / L, 8.33 mg / L and 4.16 mg / L in 24 hours of exposure. The same result was observed for the standard strain Bti001 - B. thuringiensis which showed 100% mortality at all concentrations tested. In the assay with autoclaved biomass, only the R22 line showed 90 to 100% mortality in 72 hours of exposure. In this way, the results of this work provide relevant information about the larvicidal potential of strains of isolated bacilli from different Amazonian environments, which can be used in biological control actions of Ae. aegypti. Keywords: Amazonian microbial, insecticidal proteins, hydrolytic enzymes, vector control.pt_BR
dc.languageporpt_BR
dc.publisherUniversidade do Estado do Amazonaspt_BR
dc.rightsAcesso Abertopt_BR
dc.rightsAtribuição-NãoComercial-SemDerivados 3.0 Brasil*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/br/*
dc.subjectMicrobiota amazônicapt_BR
dc.subjectproteínas inseticidaspt_BR
dc.subjectenzimas hidrolíticapt_BR
dc.subjectcontrole vetorialpt_BR
dc.titleAtividade Larvicida de Bacillus spp. da Amazônia Brasileira portadores dos genes Cry e Bsglu (β-GLUCANASE), no controle de Aedes Aegypti Linnaeus, 1762pt_BR
dc.typeDissertaçãopt_BR
dc.date.accessioned2020-03-13T14:17:18Z-
dc.contributor.advisor1Tadei, Wanderli Pedro-
dc.contributor.advisor1Latteshttp://lattes.cnpq.br/6806722604010480pt_BR
dc.contributor.referee1Tadei, Wanderli Pedro-
dc.contributor.referee1Latteshttp://lattes.cnpq.br/6806722604010480pt_BR
dc.contributor.referee2Souza, Érica Simplício de-
dc.contributor.referee2Latteshttp://lattes.cnpq.br/4333531513081697pt_BR
dc.contributor.referee3Carmo, Édson Júnior do-
dc.contributor.referee3Latteshttp://lattes.cnpq.br/5780309549588357pt_BR
dc.creator.Latteshttp://lattes.cnpq.br/7864923221950758pt_BR
dc.description.resumoO mosquito Aedes aegypti é vetor dos arbovirus Dengue, Zika e Chikungunya, os quais causam agravos à saúde pública, em diversas regiões do mundo. O controle do vetor é a melhor forma de amenizar esta problemática, que pode ser de caráter socioeducativo, físico, químico e biológico. Contudo, os produtos químicos ocasionam impactos negativos ao meio ambiente e aos seres humanos. O uso de microrganismos entomopatogênicos, principalmente bactérias do gênero Bacillus torna-se uma alternativa promissora, no combate a este mosquito. O objetivo deste estudo foi selecionar bactérias isoladas de ambientes amazônicos, portadoras dos genes Cry e BS-glu, com potencial larvicida, para contribuir no controle de populações de Ae. aegypti. Neste trabalho foram obtidas 21 linhagens de coleções de trabalhos acadêmicos, provenientes de solos, água, planta e inseto de diferentes ambientes amazônicos. As linhagens de bacilos deste estudo foram submetidas ao método de reação de PCR com a utilização da sequência de nucleotídeos que codifica para o gene rRNA16S. No total 20 linhagens bacterianas foram identificadas, pertencentes aos seguintes gêneros: Bacillus, Brevibacillus. Brevundimonas, Serratia e Achromobacter. Posteriormente, foi realizada PCR para caracterização dos genes Cry4Ba, Cry11e BS-glu. Os resultados mostraram que das 21 linhagens analisadas, seis linhagens - SPa09, SPa04, 15PHA, BtAM06, R22 e GD 02.13 - apresentaram amplificação para o gene Cry4Ba. Considerando o gene BS-glu, duas linhagens - SBC2 e cepa padrão Bti001- amplificaram para o referido gene. Na avaliação da atividade larvicida foram realizados os bioensaios quantitativos com 21 linhagens de bacilos contra larvas de Ae. aegypti. Os resultados dos bioensaios quantitativos demonstraram que sete linhagens, foram promissoras nos ensaios com a biomassa bacteriana, onde cinco - R22ISP2, GDO2.13NA, BtAM06, BtAM49LB e SPa09NA - apresentaram 100% de mortalidade em todas as concentrações - 133 mg/L, 66.6 mg/L, 33.3 mg/L, 16.6 mg/L, 8.33 mg/L e 4.16 mg/L em 24 horas de exposição. O mesmo resultado foi observado para a linhagem padrão Bti001 - B. thuringiensis que apresentou 100% de mortalidade em todas as concentrações testadas. No ensaio com biomassa autoclavada, apenas a linhagem R22 apresentou de 90 a 100 % de mortalidade, em 72horas de exposição. Desta forma, os resultados deste trabalho fornecem informações relevantes do potencial larvicida de linhagens de bacilos isolados de diferentes ambientes amazônicos, que podem ser utilizadas nas ações de controle biológico do Ae. aegypti. Palavras-chave: Microbiota amazônica, proteínas inseticidas, enzimas hidrolíticas, controle vetorial.pt_BR
dc.publisher.countryBrasilpt_BR
dc.publisher.programPós-Graduação em Biotecnologia e Recursos Naturaispt_BR
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