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dc.contributor.authorSilva, Ezequias Andrade da-
dc.date.available2020-03-12-
dc.date.available2020-03-12T15:21:16Z-
dc.date.issued2006-03-12-
dc.identifier.urihttp://repositorioinstitucional.uea.edu.br//handle/riuea/2271-
dc.description.abstractFungi belong to basidiomycete class have been confered efficiency in the biodegradation process of ligninocelullosic materials due to their enzymes. These enzymes have commercial potential that could be utilized to many industrial applications. This paper evaluate the micelial growth and enzymatic activity of laccase of three amazonic white-rot fungi (Pycnoporus sanguineus, FSF7 (Trametes sp) e FSF11 (Trametes sp). Micelial growth was evaluated through the micelial progress in solid media BDA with different concentrations (30, 90, 120 and 300 mcg) of biotin. To enzymatic activity of laccase was used liquid medium (agar-malt) with different concentrations of glucose/ yeast extract, previously autoclaved at 120 C during 15 minutes and pH 6. 5mm of fungi removed from colony edge was used to inoculate in submerged culture under agitation in Sheaker at 180 rpm and 28 C. High (2.5g) and low (25mg) dosage of seryngaldazine was used. After three days of cultivation, the media was filtrated and enzymatic activity determined by XVII spectrophotometer. P. sanguineus had higher micelial growth and FSF11lower at concentration of 120 mcg of biotin. On the whole, biotin in the medium contribute to add micelial growth. Laccase increase when use higt concentration of inductor seryngaldazine. Laccase activity to P. sanguineus increase significantly when higher dosage of seryngaldazine at 3:5 glucose/ yeast extract was used. Hence it follows that vitamin, as for example biotin, contribute to micelial growth more rapid and higher as well as a major concentration of inductor (seryngaldazine) contribute to increase laccase activity.pt_BR
dc.languageporpt_BR
dc.publisherUniversidade do Estado do Amazonaspt_BR
dc.rightsAcesso Abertopt_BR
dc.rightsAtribuição-NãoComercial-SemDerivados 3.0 Brasil*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/br/*
dc.subjectFungos ligninolíticospt_BR
dc.subjectLacasespt_BR
dc.subjectDegradação da madeira.pt_BR
dc.subjectBiotecnologiapt_BR
dc.titleAtividade Enzimática da Lacase em Três Fungos Amazônicos Degradadores de Madeirapt_BR
dc.typeDissertaçãopt_BR
dc.date.accessioned2020-03-12T15:21:16Z-
dc.contributor.advisor1Silva, Ademir Castro e-
dc.contributor.advisor1Latteshttp://lattes.cnpq.br/5162043375426666pt_BR
dc.contributor.referee1Silva, Ademir Castro e-
dc.contributor.referee1Latteshttp://lattes.cnpq.br/5162043375426666pt_BR
dc.creator.Latteshttp://lattes.cnpq.br/2477841708883982pt_BR
dc.description.resumoFungos pertencentes à classe basidiomicetos têm conferido maior eficiência no processo de biodegradação de materiais lignocelulósicos em função das enzimas que possuem para essa atividade. Essas enzimas têm potencial comercial podendo ser utilizadas em diversas aplicações industriais. O presente trabalho avalia o crescimento micelial e a atividade enzimática de lacase de três fungos amazônicos (Pycnoporus sanguineus, FSF7 (Trametes sp) e FSF11 (Trametes sp) deterioradores de madeira. O crescimento micelial foi avaliado através do avanço da fronteira micelial em meio sólido BDA, com diferentes concentrações (30, 90, 120 e 300 mcg) de biotina. Para a atividade enzimática de lacase, foi utilizado meio líquido (ágar-malte) obtendo-se diferentes concentrações de glicose/ extrato de levedura, preparado e previamente esterilizado em autoclave à 120 C por 15 minutos e pH 6. XVI Posteriormente, inoculou-se os fungos através de 5 mm de massa micelial retirados da extremidade da colônia mantendo-se a cultura submersa sob agitação em estufa Sheker a 180 rpm à 28 C. Foi utilizado meio com alta (2,5g) e baixa (25mg) dosagem de seringaldazina. Após três dias de cultivo, o meio foi filtrado e a atividade enzimática determinada em espectrofotômetro. P. sanguineus apresentou em média maior crescimento micelial em concentração de 120 mcg de biotina e FSF11 a menor média. De modo geral, a presença de biotina no meio contribuiu para o maior crescimento micelial. A produção de lacase aumentou significativamente quando se utilizou uma concentração maior do indutor seringaldazina. Para o fungo P. sanguineus a atividade de lacase aumentou significativamente quando se utilizou uma concentração maior de seringaldazina em proporção 5:3 de glicose/ extrato de levedura. Conclui-se portanto que o uso de vitaminas, como por exemplo a biotina, contribui para o crescimento micelial mais rápido e maior, assim como uma maior concentração de indutor de crescimento (seringaldazina) contribui para o aumento da atividade enzimática de lacase.pt_BR
dc.publisher.countryBrasilpt_BR
dc.publisher.programPós-Graduação em Biotecnologia e Recursos Naturaispt_BR
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