Avaliação do potencial antimicrobiano, enzimático e crescimento de um isolado amazônico do fungo Pycnoporus sanguineus Pycnoporus sanguineus.

Abstract

The development of biotechnology has unique importance for Brazil because of its low environmental impact, and the vast and almost unexplored biodiversity, especially in the Amazon region. From this perspective, the fungi have been extensively used in biotechnology processes, especially as producers of different substances of economic interest, such as enzymes, antibiotics, vitamins, amino acids and steroids. Thus this research focuses on the study of a strain of the fungus P.sanguineus (PIC- 04) Amazon that was grown in ten different culture media supplemented with regional products and agricultural waste in order to improve the production of biomass, enzymes and antimicrobial activity. The results show the largest biomass occurred in twenty-five days of growth under conditions of agitation in medium supplemented with buriti (M4), which showed biomass production of 128.4% higher than the biomass produced by the control means C1. For the antimicrobial metabolites produced in medium supplemented with buriti (M4) and in medium supplemented with orange peel with moisture (R3) showed inhibition halos above, the halos produced by the control (C1) for the strain of S. aureus (CBAM 001). As for the strain of E. coli (CBAM 001) only through R4, had antimicrobial activity comparable to control (C1). The enzyme activity was shown to be variable during the period of twenty-five days of the experiment and the highest production of laccase, peroxidase and total phenoloxidases occurred in the medium supplemented with buriti (M4) and in medium supplemented with orange peel with moisture R3 provided agitation. The results show that the tested media had a direct influence on biomass production by the fungus as well as production of enzymes and biomolecules with antimicrobial activity. KEY WORDS: Enzymes, Antimicrobial, Biomass, Culture Medium and Pycnoporus sanguineus.