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dc.contributor.authorAli, Rechfy Kasem Abou-
dc.date.available2020-03-11-
dc.date.available2020-03-11T14:04:42Z-
dc.date.issued2015-10-26-
dc.identifier.urihttp://repositorioinstitucional.uea.edu.br//handle/riuea/2206-
dc.description.abstractIntroduction: Malaria is a major public health problem, including Brazil, where the Plasmodium vivax is more prevalent. The Duffy glycoprotein acts as facilitator in the tissue invasion of erythroid by P. vivax; however, little is known about the association between DUFFY polymorphisms and malaria susceptibility caused by this type of plasmodium. New perspectives about this subject have emerged in different endemic regions also involving the parasite density infections. Objective: To investigate the impact of DUFFY polymorphisms in patients infected by Plasmodium vivax Materials and Methods: It was studied DNA samples of Patients infected by P. vivax, diagnosed in the Formation of Tropical Medicine Doctor Heitor Vieira Dourado through laboratorial tests which defined the type of malaria parasitaemia as the thick smear methods, genotyping and WBC. The verification of Duffy polymorphisms were carried out in the Amazon Blood Center, through PCR / RFLP and microarray method (Beadchip). The statistical significance of the frequencies found were analyzed using the statistical package R Core Team 2013. Results: We studied 287 patients and 273 individuals with no history of malaria. The FYBES allele was found in 53.2% of subjects with no history of malaria and 11.2% of infected patients. The FYB allele seems to be associated with middle and high parasite density. Discussion: Duffy polymorphism has been considered to be important in areas where P. vivax predominates. In the present study we confirm the FYBES allele is directly associated with a reduced expression of the glycoprotein Duffy, which may have an impact on malarial infection. Conclusion: Duffy polymorphisms may be associated as the frequency of parasite infection well as the density and the severity of the clinical manifestations.pt_BR
dc.languageporpt_BR
dc.publisherUniversidade do Estado do Amazonaspt_BR
dc.rightsAcesso Abertopt_BR
dc.rightsAtribuição-NãoComercial-SemDerivados 3.0 Brasil*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/br/*
dc.subjectMaláriapt_BR
dc.subjectPlasmodium vivaxpt_BR
dc.subjectSistema Duffypt_BR
dc.titleEstudo dos polimorfismos DUFFY em pacientes infectados com Plasmodium vivaxpt_BR
dc.title.alternativeStudy of DUFFY polymorphisms in patients infected with Plasmodium vivaxpt_BR
dc.typeDissertaçãopt_BR
dc.date.accessioned2020-03-11T14:04:42Z-
dc.contributor.advisor1Albuquerque, Sérgio Roberto Lopes-
dc.contributor.advisor1Latteshttp://lattes.cnpq.br/7446141046261325pt_BR
dc.contributor.referee1Albuquerque, Sérgio Roberto Lopes-
dc.contributor.referee1Latteshttp://lattes.cnpq.br/7446141046261325pt_BR
dc.contributor.referee2Weber, Simone Schneider-
dc.contributor.referee2Latteshttp://lattes.cnpq.br/1052122095067260pt_BR
dc.contributor.referee3Lalwani, Pritesh Jaychand-
dc.contributor.referee3Latteshttp://lattes.cnpq.br/7235381015289844pt_BR
dc.creator.Latteshttp://lattes.cnpq.br/3009339278045584pt_BR
dc.description.resumoIntrodução: A malária é um importante problema de saúde pública, inclusive no Brasil, onde o Plasmodium vivax é mais prevalente. A glicoproteína Duffy atua como facilitadora da invasão no tecido eritróide pelo P. vivax; porém, ainda pouco se conhece sobre a associação de polimorfismos DUFFY com a susceptibilidade à este tipo de malária. Novas perspectivas, sobre a associação entre esses polimorfismos e o P. vivax, têm surgido em diferentes regiões endêmicas, envolvendo também a densidade parasitária das infecções. Objetivo Investigar o impacto dos polimorfismos DUFFY na malaria causada por Plasmodium vivax Materiais e métodos: Pacientes infectados pelo P. vivax, diagnosticados na Fundação de Medicina Tropical doutor Heitor Vieira Dourado com definição do tipo de malária e parasitemia através dos métodos da gota espessa, genotipagem e leucograma. Para a verificação dos polimorfismos Duffy foram realizadas no Hemocentro do Amazonas, genotipagem através da PCR/RFLP e microarray (Beadchip). Investigamos a significância estatística das frequências encontradas foi analisada utilizando o pacote estatístico R Core Team 2013. Resultados: Foram estudados 287 pacientes e 273 indivíduos sem antecedente de malária. O alelo FYBES foi encontrado em 53,2% de indivíduos sem antecedente de malária e 11,2% de pacientes e o alelo FYB esteve mais associado à média e alta densidade parasitária. Discussão: Os polimorfismos Duffy tem se mostrado importantes nas áreas onde predomina o P. vivax. No presente estudo confirmamos que o alelo FYBES está diretamente associado com uma redução da expressão da glicoproteína Duffy, o que pode exercer um impacto na infecção malárica. Conclusão: Os polimorfismos Duffy podem estar associados tanto com a frequência da infecção quanto com a densidade parasitária e a gravidade das manifestações clínicas.pt_BR
dc.publisher.countryBrasilpt_BR
dc.publisher.programPROGRAMA DE PÓS-GRADUAÇÃO EM CIÊNCIAS APLICADAS Á HEMATOLOGIApt_BR
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dc.subject.cnpqMedicinapt_BR
dc.publisher.initialsUEApt_BR
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