Prospecção química de metabólitos secundários de fungos amazônicos e a influência de diferentes xenobióticos em seu metabolismo

Abstract

Fungi are microorganisms capable of inhabiting all places of the planet, to more extreme biomes, due to numerous adaptations stimulated by factors limiting their survival, such as temperature, soil type and humidity. This is due to their need to remain energetically transforming and interconverting various organic compounds of nature to live, grow, reproduce and build their own tissues. For this, there is a system of chemical reactions aided by enzymes that work in conjunction with the intermediate metabolism for the destination of this material through the metabolic pathways distributed in the living organism, they are the routes involved in primary metabolism and secondary metabolism. The products biosynthesized by fungi for their adaptation to the environment have already proved useful to humans in several areas of application, with this, the biotechnological advances have presented laboratory techniques to stimulate the production of these biocomposites, in particular the OSMAC. Penicillium funiculosum and Penicillium chrysogenum strains were submitted to treatments with variation of culture medium and addition of xenobiotic salts (CuSO4, MgSO4, KH2PO4 and NH4Cl) to evaluate the metabolic behavior of the samples through the profile chemistry obtained by LC-MS / MS chromatograms in positive mode. As a result, inhibition of the production of secondary metabolites by CuSO 4, such as roquerfotin C (m / z 322 [M + H] +), pistillarine (m / z 418 [M + H] +), tert. m / z 211 [M + H] +), isoquinocitrinin A (m / z 234 [M + H] +) and NH4Cl production of brocazin A (m / z 453 [M + H] +) in selected fungal strains . With this, it can be concluded that the insertion of xenobiotics in the culture, directly affected the secondary metabolism of the strains.